Composite

Part:BBa_K4060519:Design

Designed by: Cheng-Ju Lu   Group: iGEM21_NYCU-Taipei   (2021-10-21)


BBa_K4060503 + BBa_K4060508


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 657
    Illegal SpeI site found at 693
    Illegal PstI site found at 1050
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 657
    Illegal NheI site found at 144
    Illegal NheI site found at 167
    Illegal SpeI site found at 693
    Illegal PstI site found at 1050
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 657
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 657
    Illegal SpeI site found at 693
    Illegal PstI site found at 1050
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 657
    Illegal SpeI site found at 693
    Illegal PstI site found at 1050
    Illegal NgoMIV site found at 354
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construction of this part is according to igem assembly protocol.


Source

The mCherry gene and QPAS1 gene are cloned from Addgene plasmid pKA-207I10. The remaining parts are from 2019 igem kits.

References